Full Article: PDF
Scientific Object Identifier: http://s-o-i.org/1.1/TAS-12-140-21
DOI: https://dx.doi.org/10.15863/TAS.2024.12.140.21
Language: English
Citation: Abdikodirov, M.A. (2024). Development of a method for obtaining genetic lines balanced by two non-allelic Z-letals with increased quality indicators of cocoons of the silkworm bombyx mori L. ISJ Theoretical & Applied Science, 12 (140), 113-120. Soi: http://s-o-i.org/1.1/TAS-12-140-21 Doi: https://dx.doi.org/10.15863/TAS.2024.12.140.21 |
Pages: 113-120
Published: 30.12.2024
Abstract: This article presents the research results to improve the unique C-8ngl breed of the silkworm Bombyx Mori L., balanced by two embryonic Z-chromosome and sex-labeled at the egg stage. Backcross crosses were carried out using a simple genetic line with high technological properties, which refers to a genetic line that exhibits desirable traits and is easy to work with in a laboratory setting. A translocated, sex-labeled trw2w2?L-67 line was also used in the experiment. At the second stage of improvement, the analyzer was crossed using a translocation line to obtain information about which of the double genotypes was present in the resulting genotype of generations F1, F2, F3, and F4. Genetic schemes were developed to monitor the embryonic genes ?1 and ?2 attached to the Z chromosome in generations F1, F2, F3, and F4 obtained by backcrossing and to demonstrate the degree of their presence in each generation. The balance of embryonic non-allelic genes in the breeding line was achieved by obtaining the F4 generation. It was found that the newly obtained genetic line has a higher viability and quality of silk fibers than the original C-8ngl breed. This new line has passed laboratory tests and can be widely used only in food production enterprises to produce male-only hybrid combinations.
Key words: sex chromosome, silkworm, breed, genetic line, translocation, embryonic Z-lethality, reverse crossing, sex marking.
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